electroreduction of tacrolimus as immunosuppressant agent at hanging mercury drop electrode: application to determination in pharmaceutical formulation

the adsorptive behavior of the immunosuppressive agent tacrolimus was studied by cyclic and differential-pulse voltammetry on a hanging mercury drop electrode (hmde). the drug was accumulated at hmde and two well-defined peak currents were obtained at -1353 and -1417 mv vs . sce (saturated kcl) in borate buffer (ph 10.0) + 0.1 kcl solution. a voltammetric procedure was developed for the determination of tacrolimus using differential-pulse adsorptive stripping voltammetry (dpadsv). the optimum working conditions for determination of the drug were established. the analysis of tacrolimus in pharmaceutical dosage forms was carried out satisfactorily.